dapi fluorescent probe  (Thermo Fisher)

Journal: PLOS One

Article Title: Transcranial photobiomodulation therapy with 808 nm light changes expression of genes and proteins associated with neuroprotection, neuroinflammation, oxidative stress, and Alzheimer’s disease: Whole RNA sequencing of mouse cortex and hippocampus

doi: 10.1371/journal.pone.0326881

Figure Lengend Snippet: (A) WB protein bands and their normalized integrated optical density for (B) the cortex and (C) hippocampus areas. The representative fluorescence microscopy images of the brain tissue (D) before and (E) after 808 nm tPBMT, stained with Arginase1 antibody (green), iNOS antibody (red), and DAPI (blue); and (F) the relative fluorescence intensities of the antibodies. The data are presented as M ± SD ( N = 3), * P < 0.05, ** P < 0.01 for data with a statistically significant difference.

Article Snippet: The tissue slices were stained with Arginase 1 antibody, inducible nitric oxide synthase (iNOS) antibody [ ], and DAPI fluorescent probe (Thermo Fisher Scientific, USA).

Techniques: Fluorescence, Microscopy, Staining

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Pterostilbene Attenuates Cocultured BV-2 Microglial Inflammation-Mediated SH-SY5Y Neuronal Oxidative Injury via SIRT-1 Signalling

doi: 10.1155/2020/3986348

Figure Lengend Snippet: LPS induces BV-2 activation and SH-SY5Y injury in the coculture system, and PTE shows protective effects after LPS stimulation. SH-SY5Y cells were cocultured with BV-2 cells, LPS (100 ng/mL), or both BV-2 cells and LPS for 24 h, separately. (a) The morphology and viability of SH-SY5Y cells were observed under an inverted/phase-contrast microscope or using a CCK-8 assay, respectively. The BV-2 cells were preincubated with vehicle control or PTE (2.5, 5.0, or 10.0 μ M) for 2 h, followed by LPS stimulation and coculturing with SH-SY5Y cells for 24 h. The viability of (b) SH-SY5Y and (c) BV-2 cells was measured using a CCK-8 assay. (d) The LDH release in supernatants was determined using an LDH release assay and expressed as the percentage of maximum. (e) The apoptosis of SH-SY5Y cells was assessed using a TUNEL assay. The apoptotic nuclei were stained with TUNEL (green), and all nuclei were stained with DAPI (blue). (f) Apoptotic rate was computed as a percentage of the TUNEL- to the DAPI-positive nuclei. Data are shown as mean ± SEM. n = 6. α p < 0.05, compared with the control. β p < 0.05, compared with the a-BV-2. γ p < 0.05, compared with the PTE 2.5 μ M. LPS: lipopolysaccharide; a-BV-2: LPS-activated BV-2 coculture group.

Article Snippet: PTE, EX527, and DCFH-DA and DAPI fluorescent probes were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Techniques: Activation Assay, Microscopy, CCK-8 Assay, Lactate Dehydrogenase Assay, TUNEL Assay, Staining